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Non-peptide, once-per-day oral orforglipron to compete with established peptide-based, injectable GLP-1 receptor agonists

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A linear gradient of 0.1% TFA in water (Solvent A) and 0.1% TFA in acetonitrile (ACN) (Solvent B) was used, starting at 99% A with 1% B and increasing per minute at a flow rate of 1 mL/min and detection at 215 nm