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Although revascularization restores limb perfusion, it paradoxically induces oxidative stress, inflammation, endothelial dysfunction, and apoptosis, leading to tissue damage

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Store lyophilized material at −4 °F (−20 °C) or below in a sealed vial Allow the vial to reach room temperature before opening to prevent condensation Protect the peptide from prolonged exposure to light, heat, and humidity Use sterile laboratory equipment and appropriate aseptic technique during preparation Avoid repeated freeze–thaw cycles which may affect peptide stability Label all reconstituted samples with preparation date, solvent type, and concentration Following consistent handling procedures helps support reproducible results in biochemical assays and experimental model systems