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454 incorporated AcK via GCE into IRF3/IRF7 DNA-binding domain (DBD) to create single and double site-specific fully acetylated proteins and demonstrated that the introduction of an acetyl group at precise sites within the interferon (IFN) regulatory factors IRF3/IRF7 DBD region inhibits the formation of IRF3/IRF7 liquid–liquid phase separation (LLPS) and the type I IFN (IFN-I) induction