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Once the needle is in, pull back slightly on the plunger to check for blood (we call this aspiration)

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3.5 Subgroup analysis To address the considerable heterogeneity observed across studies, we performed subgroup analyses focusing on three confounding factors (animal species, treatment agents, model drugs and dosages), to identify the sources of heterogeneity for the five primary outcomes (AST, ALT, SOD, MDA, GSH)