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Research Applications and Considerations GHK-Cu liver research covers HSC activation inhibition via JNK/AP-1 and Nrf2/MMP-13 pathways, TGF-β1 Smad-independent signalling modulation, primary hepatocyte oxidative cytoprotection (H₂O₂, APAP, ethanol models), CCl₄ in vivo fibrosis attenuation with Sirius Red and hydroxyproline endpoints, NAFLD/MASLD lipid accumulation via AMPK-CPT1A-FAO, metallothionein copper buffering and ICP-MS hepatic copper quantitation, and alcohol-induced steatohepatitis antioxidant protection